Luca Comai's CV

CURRICULUM VITAE

LUCA COMAI

Professor, Plant Biology and Genome Center
UC Davis, Davis, California, USA
http://comailab.genomecenter.ucdavis.edu

EDUCATION

Ph.D., Plant Pathology, University of California, Davis, 1980

M.S., Plant Pathology, Washington State University, 1978

B.S. (equivalent), Agricultural Sciences, University of Bologna, 1976

EMPLOYMENT

2006-         Professor, Genome Center and Department of Plant Biology, UC Davis, Davis

2001-2006 Professor, Department of Biology, University of Washington, Seattle

1997-1998 Visiting Scientist, Fred Hutchinson Cancer Research Center, Seattle

1992-2001 Associate Professor, Department of Botany, University of Washington, Seattle

1990-1992 Associate Professor wot, Department of Botany, University of Washington, Seattle

1985-1989 Senior Scientist, Calgene, Inc., Davis 

1981-1985 Principal Scientist, Calgene, Inc., Davis

1980-1981 Postdoctoral Research Associate. Dept. of Plant Pathology, University of California, Davis 

1978-1980 Research Assistant, Dept. of Plant Pathology, University of California, Davis 

1977-1978 Research Assistant, Dept. of Plant Pathology, Washington State University, Pullman

SIGNIFICANT PROFESSIONAL ACTIVITIES

2017 ASPB Innovation Prize for Agricultural Technology

2016 Honorary Fellow, Institute of Advanced Studies (ISA) of the University of Bologna

2015 Distinguished Research Award, College of Biological Sciences, UC Davis

2012 Fellow of the American Association for the Advancement of Science

2012- The Arabidopsis Book, Associate Editor

2009- Arcadia Biosciences, Scientific Advisory Board

2008-2009 Board-appointment committee, iPLANT initiative

2008- Chromosoma, Associate Editor

2004 USDA-NRICGP, Functional Genomics of Crop Plants Panel  Manager

2003- 2006 Member, Seattle Cancer Alliance

2002- 2003 USDA-NRICGP, Genetics Mechanisms Panel Manager

2002- 2012 Plant Molecular Biology, Associate Editor

2001- 2002 USDA-NRICGP, Genetics Mechanisms and Genome Panel Manager

2000- 2014 Member of “Comitato Tecnico-Scientifico per la ricerca”, Province of Trento, Italy

PUBLICATIONS  (H-index= 65, 19K citations)

Maheshwari S, Ishii T, Houben A, Comai L. 2017 Centromere location in Arabidopsis is unaltered by extreme divergence in CENH3 protein sequence. Genome Research 27:471-478. doi: 10.1101/gr.214619.116. PMID: 28223399

Udomchalothorn T, Plaimas K, Sripinyowanich S, Boonchai C, Kojonna T, Chutimanukul P, Comai L, Buaboocha T, Chadchawan S. 2017 Expression in Arabidopsis of OsNucleolin1-L Enhances Photosynthesis via Transcriptome Modification under Salt Stress Condition. Plant Cell Physiol. 2017 doi: 10.1093/pcp/pcx024. [Epub ahead of print] PMID: 28204743

Akagi T, Henry IM, Kawai T, Comai L, Tao R. 2016. Epigenetic Regulation of the Sex Determination Gene MeGI in Polyploid Persimmon. Plant Cell. 28:2905-2915. doi: 10.1105/tpc.16.00532.

Jiang WZ, Henry IM, Lynagh PG, Comai L, Cahoon EB, Weeks DP. Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing. Plant Biotechnol J. doi: 10.1111/pbi.12663.PMID: 27862889 

Huo H, Henry IM, Coppoolse ER, Verhoef-Post M, Schut JW, de Rooij H, Vogelaar A, Joosen RV, Woudenberg L, Comai L, Bradford KJ. 2016 Rapid Identification of Lettuce Seed Germination Mutants by Bulked Segregant Analysis and Whole Genome Sequencing. Plant J. doi: 10.1111/tpj.13267. PMID: 27406937

Kuppu S, Tan EH, Nguyen H, Rodgers A, Comai L, Chan SW, Britt AB. 2015 Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance. PLoS Genet. Sep 9;11(9):e1005494. doi: 10.1371/journal.pgen.1005494. PMID: 26352591

Inagaki S, Henry IM, Lieberman MC, Comai L. High-Throughput Analysis of T-DNA Location and Structure Using Sequence Capture. 2015. PLoS One 10:e0139672. doi: 10.1371/journal.pone.0139672. PMID: 26445462 

Cárdenas-Conejo Y, Carballo-Uicab V, Lieberman M, Aguilar-Espinosa M, Comai L, Rivera-Madrid R. 2015 De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis. BMC Genomics 16 :877. doi: 10.1186/s12864-015-2065-4. PMID: 26511010 

Henry MI, Zinkgraf MS, Groover AT, Comai L. 2015. A system for dosage-based functional genomics in poplar. Plant Cell 27:2370-83. doi: 10.1105/tpc.15.00349

Tan EH, Henry MI, Ravi M, Bradnam KR, Mandakova T, Marimuthu MP, Korf I, Lysak MA, Comai L, S.W.L. Chan. 2015 Catastrophic chromosomal restructuring during genome elimination in plants. ELife 10.7554/eLife.06516

Burkart-Waco D, Ngo K, Lieberman M, Comai L. 2015. Perturbation of Parentally Biased Gene Expression during Interspecific Hybridization.PLoS One. 10(2):e0117293. doi: 10.1371/journal.pone.0117293. PMID: 25719202 

Maheshwari S, Tan EH, West A, Franklin FC, Comai L, Chan SW 2015. Naturally Occurring Differences in CENH3 Affect Chromosome Segregation in Zygotic Mitosis of Hybrids. PLoS Genet. 2015 Jan 26;11(1):e1004970. doi: 10.1371/journal.pgen.1004970.

Akagi T, Henry IM, Tao R, Comai L. 2014 Plant genetics. A Y-chromosome-encoded small RNA acts as a sex determinant in persimmons. Science. 346:646-50. doi:10.1126/science.1257225. PMID: 25359977 

Ravi M, Marimuthu MPA, Tan EH, Maheshwari S, Henry IM, Marin-Rodriguez B, Urtecho G, Tan J, Thornhill K, Zhu F, Panoli A, Sundaresan V, Britt AB, Comai L, Chan SWL. 2014. Nature Comm. 31 Oct 2014 doi: 10.1038/ncomms6334

Henry MI, Nagalakshmi U, Lieberman MC, Ngo KJ, Krasileva KV, Vasquez-Gross H, Akhunova A, Akhunov A, Dubcovsky J, Tai TH, and Comai L. 2014. Efficient genome-wide detection and cataloging of EMS-induced mutations using next-generation sequencing and exome capture. Plant Cell, 2014 Apr 11. PMID: 24728647

Henry MI, Dilkes BP, Tyagi A, Gao J, Chrisensen B, and Comai L. 2014. The BOY NAMED SUE quantitative trait locus confers increased meiotic stability to an adapted natural allopolyploid of Arabidopsis. Plant Cell 26(1):181-94  PMID: 24464296

Burkart-Waco D., Ngo K., Dilkes B., Josefsson C, and Comai L. 2013. Early Disruption of Maternal–Zygotic Interaction and Activation of Defense-Like Responses in Arabidopsis Interspecific Crosses. Plant Cell 25(6):2037-55. doi: 10.1105/tpc.112.108258. PMID: 23898028

Tsai, H., Missirian V., Ngo JK, Tran RK, Chan SR, Sunderesan V, Comai L.  2013 Production of a high efficiency TILLING population through polyploidization. Plant Physiol. 161:1604-14. doi: 10.1104/pp.112.213256. PMID: 23417087

*Cooper JL, Henikoff S, Comai L, Till BJ. TILLING and ecotilling for rice. 2013. Methods Mol Biol.956:39-56. PMID: 23135843

Inácio A, Pinho J, Pereira PM, Comai L, Coelho MM. 2012. Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex - The Squalius alburnoides. PLoS One 7(7):e41158. PMID: 22815952

Shi X, Ng DW, Zhang C, Comai L, Ye W, Jeffrey Chen Z.  2012. Cis- and trans-regulatory divergence between progenitor species determines gene-expression novelty in Arabidopsis allopolyploids. Nat Commun. 3:950. PMID: 22805557

Missirian V, Henry IM, Comai L, Filkov V. “POPE: Pipeline of Parentally-Biased Expression” BIOINFORMATICS RESEARCH AND APPLICATIONS - Lecture Notes in Computer Science, 2012, Volume 7292/2012, 177-188.

Seymour DK, Filiault DL, Henry IH, Monson-Miller J, Ravi M, Pang A, Comai L, Chan SWL, Maloof JN. 2012. Rapid creation of Arabidopsis doubled haploid lines for quantitative trait locus mapping. Proc Natl Acad Sci U S A, PMID: 22371599.

Monson-Miller J, Sanchez-Mendez D, Fass J,  Henry IM, Tai TH, Comai L. 2012. Reference genome-independent assessment of mutation density using restriction enzyme-phased sequencing. BMC Genomics 13(1):72 PMID: 22333298

Burkart-Waco D, Josefsson C, Dilkes B, Kozloff N, Torjek O, Meyer R, Altmann T and Comai L. 2012. Hybrid incompatibility in Arabidopsis is determined by a multi-locus genetic network. Plant Physiol. 158(2):801-12 PMID: 22135429

Tsai H, Howell T, Nitcher R, Missirian V, Watson B, Ngo K, Lieberman M, Fass J, Uauy C, Tran R, Khan AA, Filkov V, Tai TH, Dubcovsky J, Comai L. 2011. Discovery of rare mutations in populations: TILLING by sequencing. Plant Physiol. 156(3):1257-68. PMID: 21531898

Missirian V, Comai L, Filkov V. 2011. Statistical Mutation Calling from Sequenced Overlapping DNA Pools in TILLING Experiments. BMC Bioinformatics. 12:287. PMID: 21756356

Chang PL, Dilkes BP, McMahon M, Comai L, Nuzhdin SV. 2010 Homoeolog-specific retention and use in allotetraploid Arabidopsis suecica depends on parent of origin and network partners. Genome Biol. Dec 23;11(12):R125. PMID: 21182768. 

Ravi M, Kwong PN, Menorca RM, Valencia JT, Ramahi JS, Stewart JL, Tran RK, Sundaresan V, Comai L, Chan SW. 2010 The rapidly evolving centromere-specific histone has stringent functional requirements in Arabidopsis thaliana. Genetics. 186(2):461-71. Epub 2010 Jul 13. PMID: 20628040 

Hutcheon C, Ditt RF, Beilstein M, Comai L, Schroeder J, Goldstein E, Shewmaker CK, Nguyen T, De Rocher J, Kiser J. 2010 Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes. BMC Plant Biol. 10(1):233 PMID: 20977772 

Henry, I, Dilkes, BP, Miller ES, Burkart-Waco D, Comai L. 2010. Phenotypic consequences of aneuploidy in Arabidopsis thaliana. Genetics 186(4):1231-45. PMID: 20876566

Pignatta D, BP Dilkes, S-Y Yoo, I. Henry, A Madlung, R Doerge, ZJ Chen and Comai L. 2010. Differential sensitivity of the Arabidopsis thaliana transcriptome and enhancers to the effects of genome doubling. New Phyotol. 186:194 - 206

Uauy C, Paraiso F, Colasuonno P, Tran RK, Tsai H, Berardi S, Comai L, Dubcovsky J. 2009. A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat. BMC Plant Biol. 9(1):115

Walia H, Josefsson C, Dilkes B, Kirkbride R, Harada J. and Comai L. 2009 Dosage-dependent deregulation of an AGAMOUS-LIKE gene cluster contributes to interspecific incompatibility. Curr. Biol. 19:1128-32

Henry I, Dilkes B, Tyagi AP, H-Y Lin, and Comai L. 2009. Dosage and parent-of-origin effects shaping aneuploid swarms in Arabidopsis thaliana. Heredity 103(6):458-68. PMID: 19603060

Pignatta D, Comai L. 2009. Parental squabbles and genome expression: lessons from the polyploids. J Biol. 2009 May 1;8(4):43. PMID: 19439050 

Dilkes B, Spielman M, Weizbauer R, Watson B, Burkart-Waco, D, Scott R, and Comai L. 2008. The Maternally Expressed WRKY-Transcription Factor TTG2 Controls Lethality in Interploidy Crosses of Arabidopsis. PLOS Biology 6(12):2707-20  PMID: 19071961

Pignatta D, Dilkes B, Wroblewski T, Michelmore RW, Comai L. 2008. Transgene-induced gene silencing is not affected by a change in ploidy level. PLoS ONE. 2008 Aug 26;3(8):e3061. 

Cooper, JL, Till, BJ, Laport, RG, Darlow, MC, JM, Kleffner, Jamai, A, El-Mellouki, T, Liu, S, Ritchie, R, Nielsen, N, Bilyeu, KD, Meksem, K, Comai L, and S, Henikoff. 2008 Tilling to Detect Induced Mutations in Soybean. BMC Plant Biol 8: 9.

Till BJ, Cooper J, Tai T, Colowit P, Greene EA, Henikoff S, Comai L. 2007. Discovery of chemically induced mutations in rice by TILLING. BMC Plant Biology 7:19. PMID: 17428339

Henry I, Dilkes B, Comai L. 2007. Genetic basis for dosage sensitivity in Arabidopsis thaliana. PLOS Genetics 3(4):e70. PMID: 17465685

Davison J, Tyagi A, Comai L. Large-scale polymorphism of heterochromatic repeats in the DNA of Arabidopsis thaliana. BMC Plant Biol. 2007 Aug 16;7:44. PMID: 17705842

Henry I, Dilkes B, Comai L. 2006 Molecular karyotyping and aneuploidy detection in A. thaliana using quantitative fluorescent PCR. Plant J 48(2):307-19

Till BJ, Zerr T, Bowers E, Greene EA, Comai L, Henikoff S. 2006. High-throughput discovery of rare human nucleotide polymorphisms by Ecotilling. Nucleic Acids Res. 2006 Aug 7;34(13):e99.

Josefsson C, Dilkes B, Comai L. 2006. Parent-dependent loss of gene silencing during interspecies hybridization. Current Biology 16:1322-8

Ditt RF, Kerr KF, de Figueiredo P, Delrow J, Comai L, and Nester EW. 2006 The Arabidopsis thaliana transcriptome in response to Agrobacterium tumefaciens.  Mol Plant Micr Inter 19:665-81

Marfil CF, Masuelli RW, Davison J, Comai L. 2006 Genomic instability in Solanum tuberosum x Solanum kurtzianum interspecific hybrids. Genome. 2006 49:104-13.

Wang J, Tian L, Lee H-S, Wei N-E, Chen M, Lee JJ, Wang JJ, Rao S,  Jiang H, Watson B, Madlung A, Osborn TC, Doerge RW, Comai L, and Chen ZJ. 2006. Genome-wide Non-additive Gene Regulation in Arabidopsis Allotetraploids. Genetics 172:507-17

Li B, Conway N, Navarro S, Comai Luca, and Comai Lucio. 2005. A conserved and species-specific functional interaction between the Werner syndrome-like exonuclease atWEX and the Ku heterodimer in Arabidopsis. Nucleic Acid Research 33:6861-6867

Ditt R, Nester E, and Comai L. 2005 The plant defense response and Agrobacterium tumefaciens . FEMS Microbiology 247: 207-13 

Henry IM, Dilkes B, Young K, Watson B, Wu H, Comai L. 2005 Aneuploidy and genetic variation in the Arabidopsis thaliana triploid response. Genetics: 170:1979-88

Enns LC, Kanaoka MM, Torii K, Comai L, Okada K, Cleland RE.  2005 Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility.  Plant Mol Biol 58: 333-49

A Madlung, AP Tyagi, B Watson, H Jiang, T Kagochi, RW Doerge, R Martienssen and Comai L. 2005 Genomic changes in synthetic Arabidopsis polyploids. Plant Journal 41: 221-230

Lee H-S, Wang J, Tian L, Jiang H, Black MA, Madlung A, Watson B, Lukens L, Pires EJC, Wang JJ, Comai L, Osborn TC, Doerge RW, Chen ZJ. 2004 Sensitivity of 70-mer oligonucleotides and cDNAs for microarray analysis of gene expression in Arabidopsis and its related species. Plant Biotechnology Journal 2: 45-57

Comai L, Till BJ, SH Reynolds, EA Greene, C Codomo, L Enns, J Johnson, C Burtner, A Odden, K Young, and S Henikoff. 2004 Large-scale discovery of natural polymorphisms by Ecotilling. Plant Journal 37:778-786

Till BJ, Burtner C, Comai L, Henikoff S. 2004 Mismatch cleavage by single-strand specific nucleases. Nucleic Acids Res. 32:2632-41

Wang J, Tian L, Madlung A, Lee H-S, Chen M, Lee JJ, Watson B, Kagochi T,  Comai L, and Chen ZJ. 2004 Stochastic and epigenetic changes of gene expression in Arabidopsis polyploids. Genetics 167: 1961-73

Till BJ, Reynolds SH, Weil C, Springer N, Burtner C, Young K, Bowers E, Codomo CA, Enns LC, Odden AR, Greene EA, Comai L, Henikoff S. 2004 Discovery of induced point mutations in maize genes by TILLING. BMC Plant Biol. 4:12

Pontes O, Neves N, Silva M, Lewis MS, Madlung A, Comai L, Lawrence RJ, Pikaard CS and Viegas W. 2004 Chromosomal locus rearrangements are a rapid response to formation of the allotetraploid Arabidopsis suecica genome. Proc Natl Acad Sci USA 101:18240-5

Till BJ, SH Reynolds, EA Greene, C Codomo, L Enns, J Johnson, C Burtner, A Odden, K Young, N Taylor, JG Henikoff, Comai L and S Henikoff. 2003 Large-scale discovery of induced point mutations with high-throughput TILLING. Genome Research 3:524-30

Comai L, AP Tyagi, and MA Lysak. 2003 FISH analysis of meiosis in Arabidopsis allopolyploids. Chromosome Research 11:217-26

EA Greene C Codomo, N Taylor, Till BJ, SH Reynolds, L Enns, C Burtner, J Johnson, A Odden, K Young, JG Henikoff, Comai L and S Henikoff. 2003 Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164:731-40

Holmes-Davis, R and Comai L, 2002 The matrix attachment regions (MARs) associated with the Heat Shock Cognate 80 gene (HSC80) of tomato represent specific regulatory elements. Mol Genet Genomics 266:891-8 

Talbert PB, Masuelli R, Tyagi AP, Comai L, Henikoff S. 2002 Centromeric localization and adaptive evolution of an Arabidopsis histone h3 variant.  Plant Cell. 14:1053-66

Madlung A, Masuelli RW, Watson B, Reynolds SH, Davison J, and Comai L. 2002 Remodeling of DNA Methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiology 129: 733–746

Colbert TG, Till BJ, Tompa R, Reynolds SH, Steine MN, Yeung AT, McCallum CM, Comai L, and Henikoff S 2001 High-Throughput Screening for Induced Point Mutations. Plant Physiology 126:480-484

Ditt R, Nester EW, Comai L. 2001 Plant gene expression responses to Agrobacterium tumefaciens. Proc. Natl. Acad. Sci. USA. 98:10954-10959

Holmes-Davis R, Payne SR, Comai L. 2001 The effects of kinetin and hydroxyurea on the expression of the endogenous and transgenic Heat Shock Cognate 80 ( HSC80). Plant Cell Rep  20:744–748

McCallum C.M., Comai L., Greene E.A., Henikoff S. 2000 Targeted screening for induced mutations. Nature Biotechnol. 18:455-7 

Comai L., Tyagi A.P., Winter K., Holmes-Davis R., Reynolds S., Stevens Y. and Byers B. 2000 Phenotypic Instability and Rapid Gene Silencing in Newly Formed Arabidopsis Allotetraploids. Plant Cell 12: 1551-68

Deng W, Chen L, Peng WT, Liang X, Sekiguchi S, Gordon MP, Comai L, Nester EW. 1999. VirE1 is a specific molecular chaperone for the exported single-stranded-DNA-binding protein VirE2 in Agrobacterium. Mol Microbiol 31:1795-807

Deng WY, Chen LS, Wood DW, Metcalfe T, Liang XY; Gordon MP, Comai L and Nester EW. 1998 Agrobacterium VirD1 protein interacts with plant host cyclophilins. Proc. Nat. Acad. Sci. USA 95: 7040-7045.

Henikoff S and Comai L.1998. A DNA methyltransferase homolog with a chromodomain exists in multiple polymorphic forms in Arabidopsis. Genetics 149: 307-318.

Chen J, Comai L, Pikaard C. 1998 Gene dosage and stochastic effects determine the severity and direction of uniparental rRNA gene silencing (nucleolar dominance) in Arabidopsis allopolyploids. Proc. Nat. Acad. Sci. USA.95 :14891-14896. 

Chinn, A., Payne, S.R., and Comai L. 1996 Variegation and silencing of the Heat Shock Cognate 80 gene are relieved by a bipartite downstream regulatory element. Plant Journal 9: 325-339

Chinn, A. and Comai L. 1996  The Heat Shock Cognate 80 gene is flanked by nuclear matrix attachment regions.  Plant Molecular Biology 12: 959-968

Masuelli R, Tanimoto E, Brown C and Comai L. 1995 Irregular meiosis in a somatic hybrid between S.bulbocastanum and S.tuberosum detected by species-specific PCR markers and cytological analysis. Theoretical and Applied Genetics 91: 401-408

Talbert, P, Adler HT, Parks, D and Comai L. 1995 The REVOLUTA  gene is necessary for apical meristem development and for limiting cell divisions in the stems and leaves of Arabidopsis. Development 121: 2723-2735

Kovacic, R.T., Comai L. and Bendich, A.J. 1995 Protection of megabase DNA from shearing. Nucleic Acid Research  23: 3999-4000

Tanimoto EY, Rost, LR and Comai L 1993 Replication-dependent histone H2A mRNA expression in pea root tips. Plant Physiology 103: 1291-129

Koning, A, Jones A., Fillatti, J, Comai L and Lassner, MW. 1992 Arrest of embryo development in Brassica napus mediated by modified Pseudomonas aeruginosa exotoxin A. Plant Molecular Biology 18: 247-258

Koning A, Rose, R and Comai L. 1992 Developmental regulation of tomato Heat Shock Cognate protein 80 gene. Plant Physiology 100: 801-811

Lassner MW, Jones A, Daubert S and Comai L. 1991 Targeting of T7 RNA polymerase in tobacco nuclei mediated by an SV40 nuclear location signal. Plant Molecular Biology 17: 229-234

Koning A, Tanimoto EY, Kiehne K, Rost T. and Comai L. 1991 Cell specific expression of plant histone H2A genes. The Plant Cell 3: 657-665

Comai L, Maslyar D and Moran P. 1990 Novel and useful properties of a chimeric plant promoter combining CaMV 35S and MAS elements. Plant Molecular Biology. 15: 373-381

Comai L., Larson-Kelly N., Kiser J, Mau, C., Pokalsky AR, McBride KE, Shewmaker CK and Stalker DM 1988 Chloroplast delivery of a RuBP carboxylase small subunit-EPSP synthase chimeric protein requires a portion of the mature small subunit protein in addition to the transit peptide. J. Biological Chemistry 263: 15104-15109

Fillatti JJ, Kiser J, Rose R and Comai L. 1987 Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Bio/Technology 5: 725-730.

Fillatti, J.J., Sellmer, J., McCown, B., Haissig, B. and Comai. L. 1987 Agrobacterium mediated transformation and regeneration of Populus. Mol. Gen. Genet. 206: 192-199.

Comai L, Facciotti D, Hiatt W, Thompson G, Rose R and Stalker D. 1985 Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate.  Nature 317: 741-744

Stalker, D., Hiatt, W. and Comai L. 1984 A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. J. Biol. Chem. 260: 4724-4728.

Surico G, Comai L and Kosuge T. 1984 Pathogenicity of strains of Pseudomonas syringae pv. savastanoi and their indoleacetic acid-deficient mutants on olive and oleander. Phytopathology 74: 490-493.

Comai L. and Kosuge, T. 1983 Transposable element that causes mutations in a plant pathogenic Pseudomonas sp. J. Bacteriol. 143:950-957.

Comai L., Schilling-Cordaro, C., Mergia, A., and Houck, C.H. 1983 A new technique for genetic engineering of Agrobacterium Ti plasmid. Plasmid 10: 21-30.

Comai L., Sen, L. and Stalker, D. 1983 An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221: 370-371.

Comai L. and Kosuge, T. 1982 Cloning and characterization of iaaM, a virulence determinant of Pseudomonas savastanoi. J. Bacteriol. 149:40-46.

Comai L., Surico, G., and Kosuge, T. 1982 Relation of plasmid DNA to indoleacetic acid production in different strains of Pseudomonas syringae pv. savastanoi. J. Gen. Microbiol. 128:2157-2163.

Comai L. and Kosuge, T. 1980 Involvement of plasmid deoxyribonucleic acid in indoleacetic acid synthesis in Pseudomonas savastanoi. J. Bacteriol. 143:950-957.

INSTRUCTIONAL VIDEOS

GENETICS: https://www.youtube.com/channel/UCaex3R9Oeuui45yAxdvDj9Q

Examples:

• Genetic epistasis. 2016. https://www.youtube.com/watch?v=0HfkCppZLfo&t=3s
• Complementation and recombination in bacteriophages. 2016. https://www.youtube.com/watch?v=-bAHzabjVUs
• LOD mapping with pedigrees, part I. 2016. https://www.youtube.com/watch?v=-8ZnP1XCPeE
• LOD mapping with pedigrees, part II. 2016. https://www.youtube.com/watch?v=BwhM3PfnP-c

REVIEWS, PERSPECTIVES, BOOK CHAPTERS, ISSUED PATENTS

Comai L, Maheshwari S, Marimuthu PM. 2017. Plant centromeres. Curr. Opinion Plant Bio (in press)

Tsai H, Ngo K, Lieberman M, Missirian V, Comai L. 2015 Tilling by sequencing. Methods Mol Biol. ;1284:359-80. doi: 10.1007/978-1-4939-2444-8_18.

Comai L. 2014. Genome elimination: translating basic research into a future tool for plant breeding. PLoS Biol, 12 (6), e1001876. PMID: 24915001

Comai L. Human genetics: Human genetics discovering ourselves. Heredity. 2007 PMID: 17878919 

Woodhouse, M., Burkart-Waco, D. & Comai, L. 2009 Polyploidy. Nature Education 2(1)

Till BJ, Zerr T, Comai L, Henikoff S. 2007. A protocol for TILLING and Ecotilling in plants and animals. Nat Protoc. 2006;1(5):2465-77. 

Till BJ, Colbert T, Codomo C, Enns L, Johnson J, Reynolds SH, Henikoff JG, Greene EA, Steine MN, Comai L, Henikoff S. 2006. High-throughput TILLING for Arabidopsis. Methods Mol Biol. 2006;323:127-35. 

Comai L and S Henikoff. 2006. TILLING: practical single nucleotide mutation discovery. Plant Journal 45:684-94

Slade AJ,  Madisen L, Comai L. 2006. Compositions and methods for modulation of plant cell division. US patent # 20,060,031,967.

Comai L and R Cartwright.  2005. The toxic mutator hypothesis for hothead reversion. Plant Cell 17:2856-8

Comai L. 2005. Advantages and disadvantages of polyploidy.  2005 Nature Reviews Genetics 6:836-846

Wang J, Lee JJ, Tian L, Wang JJ, Lee H-S, Chen M, Rao S,, Wei N-E, Doerge RW, Comai L, and Chen ZJ. 2005 Methods for genome-wide analysis of gene expression changes in polyploids. Methods in Enzymology 395:570-596

Henikoff S, Till BJ, Comai L. 2004. TILLING. Traditional mutagenesis meets functional Genomics. Plant Physiol. 135:630-6

Madlung A, Comai L. 2004 The effect of stress on genome regulation and structure. Ann Bot 94(4):481-95

Dilkes PB, Comai L. 2004 A Differential dosage hypothesis for parental effects in seed development.  Plant Cell 16:3174–3180 

Osborn TC, Pires JC, Birchler JA, Auger DL, Chen ZJ, Lee HS, Comai L, Madlung A, Doerge RW, Colot V, Martienssen RA. 2003 Understanding mechanisms of novel gene expression in polyploids. Trends Genet. 19:141-7

Henikoff S and Comai L. 2003 Single nucleotide mutations for functional genomics Ann Review Plant Biology 54: 375-401

Comai L, A Madlung, C Josefsson and A Tyagi. 2003 Do the different parental ‘heteromes’ cause genomic shock in newly formed allopolyploids? Philos Trans Royal Soc 358:1149-55

Till BJ, Colbert T, Tompa R, Enns L, Codomo C ..et al…. Comai L and Henikoff S. et al., 2002 High-throughput TILLING for functional genomics, pp. in Plant Functional Genomics: Methods and Protocols, edited by E. Grotewald. Humana Press.

Goodman RM, Knauf CV. Houck CM, and Comai L. 2001 Molecular farming. [1990 #4,956,282]  # 20,010,049,830

McCallum C.M., Comai L., Greene E.A., Henikoff S. 2000 Targeting Induced Local Lesions IN Genomes (TILLING) for plant functional genomics. Plant Physiology 123(2):439-42

Comai L. Genetic and epigenetic interactions in allopolyploid plants. 2000 Plant Mol. Biol. 43: 387-399

Comai L., Sanger, M., Daubert S. 2000. Figwort mosaic virus promoter and uses. US Patent 6,051,753

Comai L. 1998 Botanica (a review of recent advances in plant molecular biology). Enciclopedia Italiana 10 (suppl. II) 162-169

Holmes-Davis R; Comai L. 1998 Nuclear matrix attachment regions and plant gene expression. Trends in Plant Science 3 (3): 91-97. 

Henikoff S; Comai L. 1998 Trans-sensing effects: The ups and downs of being together. Cell 93: 329-332.

Chinn, A. and Comai L.  1996  Transcription. In:  Transgenic Plants: a production system for industrial and pharmaceutical proteins.  M.R.L. Owen  and J. Pen eds. Wiley and Sons. Chichester

Tanimoto E. Y., Rost L.R., and Comai L. 1993 Histone H2A expression during S-phase: histological co-localization of H2A mRNA and DNA synthesis in pea root tips. In: Molecular  and cell Biology of the Plant Cell Cycle. J.C. Ormrod and D. Francis eds. Kluwer, Dordrecht. pp85-96.

Comai L. 1993 Impact of plant genetic engineering on food and nutrition. Ann. Rev. Nutr. 13:191-215

Comai L. and Koning, A.J. 1993 Plant proteins, promoters, coding sequences and use. # 5,187,267. 34 pages

Comai L. and Moran, P. 1992. CAMV35S-enhanced mannopine synthase promoter and method for using same. # 5,106,739. 21 pages

Comai L. 1991. Genetic engineering of extrachromosomal elements, plasmids and prokayotic microorganisms. US Patent 5,068,193,-

Comai L. 1990 Ti plasmid vectors for transformation of plants. pp 520-525. In: Methods in Phytobacteriology. Z. Klement, K. Rudolph, and D.C. Sands eds. Akademiai Kiado, Budapest.

Comai L., Larson-Kelly, N., Kiser, Jones, A., C., Stalker, D.M., Moran, P., Kiehne, K., Koning, A. 1989 Genetic engineering of plants for herbicide resistance. Expression of a RuBP carboxylase small subunit-EPSP synthase chimeric gene, chloroplast transport of the hybrid protein, and tolerance phenotype of transgenic plants. Vortr. Pflaenzenzuechtg. 16:441-454

Fillatti, J. and Comai L. 1989 Transformation and foreign gene expression with woody species. # 4,795,855. 14 pages.

Comai L. 1988 Novel method and compositions for introducing alien DNA in vivo. #4,762,785. 8 pages.

Hiatt, W.R., Sheehy, R.E. and Comai L. 1987 The application of genetic engineering to crop improvements. Proceedings of the Oregon Horticultural Society, Jan. 27-29, 1987, Portland, OR. Vol. 78, pp. 13-14.

Thompson, G.A., Hiatt, W.R., Facciotti, D., Stalker, D.M. and Comai L. 1987 Expression in plants of a bacterial gene coding for glyphosate resistance. Weed Sci. 35:19-23.

Comai L. and Stalker, D. 1986 Mechanisms of herbicide resistance and their manipulations. In: Oxford Surveys of Plant Molecular and Cellular Biology. Vol. 3. B.J. Miflin ed. Oxford University Press.

Comai L. 1985 Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use. #4,769,061. 22 pages.

Hiatt, W., Comai L., Huang, L-J., Rose, R., Thompson, G. and Stalker, D. 1985 Introduction and expression in plants of a glyphosate resistant aroA gene isolated from S. typhimurium. In: Molecular Form and Function of the Plant Genome. L. VanVioten- Doting, G.S.P. Groot and T. Hall eds. Plenum Press, pp. 479-488.

Comai L., Hiatt, W., Facciotti, D., Rose, R., Thompson, G. and Stalker. D. 1985 Introduction and expression in plants of a glyphosate resistant aroA gene isolated from S. typhimurium. In: Biotechnology in Plant Science: Relevance to Agriculture in the Nineteen Eighties. M. Zaitlin, P. Day, A. Hollaender eds. Academic Press, Orlando, FL, pp. 329-338.

Comai L. and Stalker, D. 1984 Impact of genetic engineering on crop protection. Crop Protection 3: 399-408

Comai L. and T. Kosuge. 1983 The genetics of indoleacetic acid production and virulence in Pseudomonas savastanoi. In: Molecular Genetics of the Bacteria-Plant Interaction. A. Puehler (ed.). Springer Verlag, Berlin.

Kosuge, T., Comai L. and Glass, L. 1983 Virulence determinants in plant pathogen interactions. In: Plant Molecular Biology. R.B. Goldberg ed.

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